One of the two major components of milk, whey is the liquid portion of the milk that separates from the curds (the other major component), during the process of making cheese. Whey contains proteins, fats, carbohydrates, vitamins, and minerals.

Due to its strong amino acid profile and ease of absorption, it is arguably the most popular sports nutrition supplement. Whey protein has been shown to augment muscle protein synthesis, support fat burning, boost the immune system, improve insulin sensitivity, and decrease appetite. Additionally, whey concentrate is shown to boost production of glutathione, the body’s master antioxidant.1

Whey protein exists in three main forms: isolate, concentrate, and hydrolysate (where whey isolate undergoes additional processing.). This article will focus on the differences between whey protein concentrate and isolate. Both forms contain whey protein, but whey isolate, which is made from whey concentrate, undergoes further processing which yields an end product with more protein per unit than whey concentrate. This processing results in a product that differs from whey concentrate in several important ways.

How is Whey Protein Made?

Pushing the liquid portion of milk through a filter creates whey protein. The material left behind is dried and forms whey protein concentrate. Concentrate contains varying amounts of fat and carbohydrates in the form of lactose. The percentage of protein varies from about 30% to about 80%, and includes a variety of protein subfractions,2 many of which have significant biologic activity and health benefits. Evidence suggests that these peptides must remain in their native, undenatured form (the 3-d form that exists in nature) in order to exert these properties.

Why is this Important in Whey Concentrate vs Isolate?

As whey concentrate is further processed and purified into whey protein isolate,3 these 3-d structures can degraded and lose their biologic activity. You should note, however, that the amino acid sequences do not change when protein is denatured, and whether a protein is denatured during processing does not affect its muscle-building qualities. All large proteins are broken down during digestion into smaller protein chains and individual amino acids (denatured), and whether this process occurs in the gut or in the manufacturing plant is irrelevant to the muscle fibers getting these proteins.

However, since denaturing can affect the biologic activity of certain peptides, whey concentrate has a theoretical health advantage over isolate. That being said, depending on the process used, whey isolate may still have significant amounts of bioactive peptides. Ion-exchange is a purification process that, while producing the highest concentration of protein, essentially eliminates all bioactive compounds. Micro-filtration techniques, such as Cross Flow Micro filtration, are a more expensive procedure but yield a whey isolate with more intact bioactive peptides. Hydrolyzed whey isolate is whey isolate that has been further broken down, yielding small peptides that are rapidly absorbed into the blood stream. Again, though, this extra processing comes at the cost of destruction of health promoting substances.

So, Which Type of Whey Should You Chose?

When choosing a whey protein product, it is important to consider your goals, budget, and any allergies. For example, since whey concentrates contain significant amounts of lactose, anyone with lactose intolerance should avoid them. Since isolates undergo more processing, they are more expensive and can lose many health-promoting compounds found in concentrates; on the flip side, they contain a higher amount of protein per serving. This is important for people who are calorie restricting and want to consume as much real food as possible, and while 5 grams of carbs (per serving of whey concentrate) may not seem like a lot, 2 shakes a day can mean a half of an apple. Whey isolates, and particularly hydrolysates, are more rapidly absorbed than concentrates and create a more profound insulin response.4 This makes whey isolates popular post-workout choices. Whether this increased rate of absorption translates into any real-world anabolic advantage is arguable, but anyone wishing to limit rises in insulin may want to avoid isolates because of their effects on insulin release.

My recommendation: If you want a general protein supplement that will help improve your health and not break the bank (and are not lactose intolerant), pick a whey protein concentrate that is at least 80% protein. True Nutrition makes a solid whey concentrate. If you want a pure, rapidly absorbed whey for post workout that’s easy on the stomach and you are not as concerned about other health benefits and price, try an isolate, or hydrolyzed whey isolate. My pick here is Dymatize Iso-100.

Hope this helps you find the right type of whey for you and your goals.

And if your goal is the get lean and strong, check out BuiltLean’s 12-Week Body Transformation Program.

Show 4 References

  1. Ha E, Zemel MB. Functional properties of whey, whey components, and essential amino acids: mechanisms underlying health benefits for active people (review). J Nutr Biochem. 2003;14(5):251-8.
  2. These include lactoferrin, immunolgobulins, beta-lactoglobulin, alpha-lactalbumin, glycomacropeptides and bovine serum albumin.
  3. Once a whey product surpasses 85% protein content, it becomes whey protein isolate.
  4. Frid AH, Nilsson M, Holst JJ, Björck IM. Effect of whey on blood glucose and insulin responses to composite breakfast and lunch meals in type 2 diabetic subjects. Am J Clin Nutr. 2005;82(1):69-75.


  1. sylvain May 14, 2013 - 21:28 #

    Dymatise like many other brands put artificial stuff ,GMO products, high fructose corn syrup and a lot of shit in their “”Healthy product” I would not recommend those to anybody anymore. Let’s call it Junk fitness food
    Do you have any product choice that would be : No sweet , unflavored, uncolored, and organic if possible, non GMO for vegans (peas, hemp protein).
    Thank you ,

  2. Alexander May 14, 2013 - 23:33 #

    Thank you Dr. Seltzer for sharing. Interesting to hear about whey protein isolate/concentrate from a medical perspective. In the absence of whey protein, would low-fat milk be a good subsitute for post-workout? I’ve always thought that the mixture of whey and casein would result in both fast and sustained absorbtion of protein into the body and hence be good.

    1. Charlie Seltzer May 15, 2013 - 08:49 #

      Yes, Alexander. Low-fat or skim milk is a very solid post workout choice. While I think whey is the best post workout choice if you can eat a whole food meal within an hour or two, if you cannot, a mix of faster and slower proteins is absolutely a better option. And even if you do eat soon after completing a workout, low-fat milk will still work very nicely.

  3. Jerry Jun 04, 2013 - 12:04 #

    Many whey supplements recommend a daily dosage limit. Is this because of a general recommend upper limit of daily protein intake, or is this limit due to something in the whey product itself?

    1. Charlie Seltzer, MD Jun 05, 2013 - 07:58 #

      Jerry- There is no reason for a healthy person (i.e. without liver or kidney issues) to limit protein intake. Of course, someone may want to limit intake of whole-food proteins sources with also contain significant amounts of carbs or fats, but protein by itself will cause no harm to healthy livers and kidneys. I hope this helps.